Senior Scientist – Gene Therapy & CAR-T (Project Lead, CART-AI Platform) (m/f/d)

108_605

Obowiązki

• Oversee the scientific direction, planning and execution of the CARTAI project, ensuring alignment with grant objectives and timelines. 
• Coordinate experimental work across multiple domains, including genetic engineering, CAR-T construct development and cell-based functional assays (including cytotoxicity and avidity studies). 
• Lead scientific troubleshooting, data interpretation and decision making across project stages. 
• Ensure high quality reporting, documentation, and compliance with grant requirements and internal QA standards. 
• Guide the design, generation and optimization of CAR constructs, including scFv engineering, promoter/element selection and lentiviral vector strategies. 
• Supervise or perform laboratory work related to:  

• Genetic modification of T cells and reporter lines. 
• High-throughput selection of CAR variants (fluorescent reporter assays, library screening) 
• In vitro cytotoxicity, binding/avidity assays, and kinetic functional testing of CAR-T cells.
• Transduction, expansion and characterization of primary human T cells.
• Integration and interpretation of AI/ML derived CAR sequence predictions together with wet lab results in collaboration with AI team. 

• Act as the main scientific point of contact for internal stakeholders, project managers and senior leadership. 
• Collaborate with computational biology, machine learning, in vitro pharmacology, HTS, and in vivo teams to ensure seamless execution of complex workflows. 
• Contribute to scientific communication, risk mitigation, publication planning and preparation of future grant extensions. 

Wymagania

• PhD in molecular biology, immunology, biotechnology, gene therapy, or related field with 2+ years of postdoctoral/industry experience. 
• Strong, demonstrable expertise in:  

• Molecular genetics & gene engineering (cloning, vector design, lentiviral systems). 
• Gene therapy modalities and genetic modification of immune cells. 
• CAR-T biology, including CAR architecture, signaling domains, functional readouts and T-cell biology. 

• Proven ability to lead complex, interdisciplinary research projects. 
• Hands-on experience with:  
  • Engineering and production of viral vectors (lentiviral/retroviral). 
  • Mammalian cell culture, primary T-cell isolation/activation, and transduction. 
  • Assay development for cell-based reporter systems, cytotoxicity, and receptor functionality. 
  • Flow cytometry, qPCR, Western blot, and fluorescence-based readouts. 
• Excellent project planning, documentation and communication skills. 
• Ability to supervise, mentor and coordinate work across multiple scientific teams. 
• Strong analytical mindset and ability to work independently in a dynamic R&D environment. 
• Experience collaborating with AI/ML teams or interpreting computational predictions is optional. 
• Knowledge of Lumicks (z-Movi, avidity) or similar technologies will be an advantage. 

Oferujemy

• A leading scientific role in a high-impact, strategic European-funded CAR-T innovation project. 
• Opportunity to shape a next-generation platform integrating genetic engineering, advanced cell biology and artificial intelligence. 
• Work in a modern research environment with state-of-the-art equipment and a multidisciplinary team. 
• Professional growth within one of Europe’s fastest-growing biotechnology R&D organizations. 
• Additional benefits: a prepaid lunch card, private medical care, subsidized sports card, and office fruit provision.
• Internal Development initiatives including soft & leadership skills training programs.
• Recognition Program.
• Employee Referral Program.
• Support & incentive bonus for completing Ph.D.
• Various sports and engagement initiatives.

Źródło: Selvita/Praca