Scientist - Recombinant Protein Production & Cell-Based Assays (m/f/d)

108_612

Obowiązki

• Design and optimize expression of recombinant proteins, antibodies and VHH in bacterial and mammalian systems.
• Perform transient transfections and support development of stable expression systems.
• Execute small- to mid-scale protein production.
• Purify proteins using affinity, ion exchange and size exclusion chromatography (ÄKTA/FPLC experience is a plus).
• Perform protein characterization using SDS-PAGE, Western blot and SEC-HPLC.
• Maintain adherent and suspension mammalian cell lines.
• Develop and perform cell-based functional assays (e.g. proliferation, apoptosis).
• Experience with PBMCs or primary blood cells is an advantage.
• Analyze biological data and troubleshoot basic assay variability.
• Plan and execute experiments in line with project goals.
• Maintain accurate laboratory documentation and records.
• Prepare technical reports and participate in project meetings.

Wymagania

• MSc or PhD in Biotechnology, Biology, Molecular Biology, Biochemistry, Cell Biology, or a related field.
• Minimum 3 years of hands-on experience in recombinant protein production in academic or industry settings.
• Experience with recombinant protein or antibody production across multiple expression systems.
• Practical knowledge of chromatographic purification techniques and mammalian cell culture.
• Experience in developing and performing cell-based assays.
• Familiarity with protein analytical methods, including ELISA-based assays.
• Strong data analysis and interpretation skills.
• Good command of English, both written and spoken.
• Experience working in a client-facing CRO environment is an advantage.
• Ability to work effectively in cross-functional project teams.

Oferujemy

• Working in world-class extensive research facilities and modern laboratories.
• Daily cooperation and know-how exchange with scientific experts.
• Additional benefits: a prepaid lunch card, private medical care, subsidized sports card, and office fruit provision.
• Internal Development initiatives including soft & leadership skills training programs.
• Recognition Program.
• Employee Referral Program.
  
  
• Support & incentive bonus for completing Ph.D.
  
  
• Various sports and engagement initiatives.

Źródło: Selvita/Praca